Isaac married Mary F. Pyles in 1866. Mary (daughter of Thomas Pyles and Julia Ann Wilson) was born on 20 May 1846 in Kentucky, United States; died on 4 Apr 1926 in Robertson County, Kentucky, United States; was buried on 6 Apr 1926 in Piqua Cemetery, Piqua, Robertson, Kentucky, United States. [Group Sheet] [Family Chart]

1. Wilmer Forrest Chamberlin was born on 12 Oct 1867 in Robertson County, Kentucky, United States; died on 9 Jul 1946 in Lexington, Fayette, Kentucky, United States; was buried on 11 Jul 1946 in Piqua, Robertson, Kentucky, United States.
2. Bertha B Chamberlin was born in Jul 1871; and died.
3. Ernest Tecumsu Chamberlin was born on 12 Mar 1873; died on 21 Oct 1945 in Lexington, Fayette, Kentucky, United States; was buried on 23 Oct 1945 in Piqua Cemetery, Piqua, Robertson, Kentucky, United States.
4. Clarence Chamberlin was born in Mar 1875; died on 10 Dec 1957 in Robertson County, Kentucky, United States.
5. William H Chamberlin was born in Sep 1878; and died.
6. Ragan E Chamberlin was born in Mar 1885; and died.
7. Taylor B Chamberlin was born in Jun 1890; and died.